Journal: Bioactive Materials

Article Title: Commensal microbiota–coated biohybrid implants induce antibiofilm, osteogenic, and immunomodulatory responses in a human 3D immunocompetent model

doi: 10.1016/j.bioactmat.2026.06.006

Figure Lengend Snippet: Commensal microflora coatings support adhesion and viability of human cells. (A) CLSM images showing the morphology and distribution of gingival fibroblasts, oral keratinocytes, and macrophages after 48 h on glass, Ti, and CHM. Scale bar: 100 μm. (B) Metabolic activity of (a) fibroblasts, (b) keratinocytes, and (c) macrophages after 48 h on the indicated surfaces. Cells were stained with green fluorescent Calcein AM to label viable cells and with red fluorescent propidium iodide (PI) to label nonviable (dead) cells. All experiments were conducted using three biological replicates (N = 3) and three technical replicates per group (n = 3). For CLSM, the biocompatibility assay was imaged at 10× magnification with a voxel size of 1.52 μm (x) × 1.52 μm (y) × 3 μm (z).

Article Snippet: Ti- and CHM-integrated tissues, after coculture under sterile or biofilm-infected conditions, were longitudinally sectioned at the tissue–implant interface and stained with Calcein AM and propidium iodide (Invitrogen, Germany) for 30 min.

Techniques: Activity Assay, Staining

Journal: ACS Nano

Article Title: Carrier-Free Nanoassembly Suppresses Phase Separation via Ribosome-Inspired Crowding Control for Enhanced Chemo-Immunotherapy

doi: 10.1021/acsnano.5c17081

Figure Lengend Snippet: Mechanism of tumor growth inhibition by OSI-027. (A) Apoptosis of PDAC cells by the OSI-027-treated THP-1-cell system and (B) quantification of the positive rate of apoptosis as a percentage. (C) Phagocytosis of PDAC cells by the OSI-027-treated THP-1-cell system and (D) quantification of the positive rate of phagocytosis as a percentage. (E) A schematic of the HUVEC culture supernatant of the OSI-027-treated coculture system. (F) Microvascular density of the PDAC cell and THP-1-cell coculture system after OSI-027 treatment and (G) quantification of the area ratio of the microvascular density percentages. Data are shown as mean ± SD ( n = 3). (* p < 0.05, ** p < 0.01 and *** p < 0.001).

Article Snippet: The mTOR inhibitor (OSI-027) and Annexin V/propidium iodide (PI) apoptosis assay kits were purchased from MedChemExpress (NJ, USA).

Techniques: Inhibition